By shirishpatel on Friday, April 6, 2001 - 05:45 am:
is it possible to analysed acarbose which is well known antidiabets by hplc and uv detection.
By Merlin on Monday, April 9, 2001 - 11:01 am:
Acarbose is a sugar with an unconjugated double bond and a secondary amine. Both of these functionalities have some (albeit poor) UV absorption, so it should be detectable at a wavelength of <210 nm. Use an amino column, with MeCN/water mobile phase. Start with 75/25. If retention is too long, increase water to 30%. If retention is too short, decrease water to 20%. Your best bet for detection though, would probably be with an ELS detector.
By Anonymous on Tuesday, April 10, 2001 - 01:43 pm:
Hello, Merlin:
Is this HILIC? Thanks a lot for your answer in advance.
By SHIRISHPATEL on Tuesday, April 10, 2001 - 10:00 pm:
I STARTED WITH THE SAME SYSTEM AND GOT THE PEAK AT AROUND 5.4 MINUTES BUT IT WAS SPLITTING SO I THOUGHT THAT EPIMERS ARE SEPERATING SO I INCREASED THE TEMP TO 40 AND GOT THE GOOD SHAPE PEAK BUT THE IMPURITY AT THE FRONT AND BAKC OF MAIN PEAK ARE VERY DIFFICULT TO SEPERATE I KNOW THAT RI OF ELECTRON LIGHT SCATTERING DETECTOR CAN WORK BEST FOR THIS APPLIOCATION BUT THIS IS MY LIMITATIONS.
By nasrinrezanour on Wednesday, July 7, 2004 - 09:30 am:
is it possible to analysed topiramate by hplc and uv detection
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