By Anonymous on Friday, April 6, 2001 - 02:38 pm:
Does anyone know how to prepare a cyanocobalamin sample in a multivitamin tablet with minerals and herbs? Method of analysis is HPLC. Thanks
By Ana Guillén on Thursday, May 24, 2001 - 08:40 am:
Try extracting it in methanolic solution at 90 °C
Methanolic solution: 2.0 g Titriplex(Merck)+ 850 ml destilled water + 150 ml Methanol + 2.5 ml ortophosphoric acid; stirr (at least) for 7 hours.
Extraction: weigh the sample amount necessary for the assay in a volumetric frasc of 100 ml. Add 50 ml methanolic solution; heat for 10 minuts at 90 °C, shaking vigoursly every 2-3 minutes; allow to cool and fill until 100 ml with the methanolic solution.
Apply to your HPLC sistem
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