By Gregg on Friday, April 6, 2001 - 03:27 pm:
I am having carry over problems with a protein HPLC method (up to 2%). I have tried washing the column extensively in the strong solvent, which minimizes the problem, but the carry over never goes away. Is 2% carry over unheard of in protein analysis? Is there anywehere in the USP or ICH guidlines that address this issue?
By Anonymous on Saturday, April 7, 2001 - 07:10 am:
There were discussions last September on this topic in this forum. You should check them. As discussed from that discussion, the carryover is not likely from the column. Instead it may likely come from the autosampler (valve or sample loop). Good luck!
By Anonymous on Saturday, April 7, 2001 - 03:04 pm:
Is this a reversed-phase method? Ion-exchange? SEC?
By Gregg on Monday, April 9, 2001 - 06:55 am:
It is a reversed phase method. I've done multipule blank injections, with the carry over decreasing each injection ( carry over is gone after 4 blank injections). I'm using a Vydac C4 column. The gradient contains 0.05% TFA in ACN and H2O.
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