Hello,

Who can me help to set up a HPLC for analysis of organic acids, aldehydes and ketones?
Where can I find some informations.

It may not be practical (or even possible) to have a single method that will handle all three analyte types. We use a Supelco gel column (610H)with a 0.1% phosphoric acid mobile phase and UV detection for organic acids. Check the Supelco catalog for details. We use DNPH derivitization with UV detection for aldehydes. For ketones, we normally use GC, but reversed phase LC with a C8 or C18 column and UV detection will work. Check the chromatography supply catalogs for specific method parameters.

I use the LC-MS 1100 and have separated all three types of analytes in a single run using an XDB C8 and C18. Try a gradient, I have used 10-95% ACN+0.1%formic acid and have achieved good separation of all three in a single run. However, remember that if your using a Mass Spec some acids such as COOH will ionize in negative mode only. I find for these analytes UV detection at 210nm or 254nm is most accurate.