Does anybody do HPLC on uronic acids and monosaccharides in the same sample? Which colums, flow rates, mobile phase, etc. do you apply? We would like to determine both compounds in one run. Thank you for your help!

These two classes of analytes can be analyzed simultaneously using high pH anion exchange chromatography. I found a reference "The use of HPAE-PAD for the analysis of thickening agents in fruit juice and food analysis", Koswig, S., Fuchs, G., Hotsommer, H. J., Seminars in Food Analysis 2, 71-83 (1997) published by Chapman and Hall.

The method utilizes an isocratic portion for the analysis of the monosaccharides using 0.02 molar sodium hydroxide followed by a sodium acetate gradient to 0.225 molar sodium acetate (holding the hydroxide concentration constant)which elutes the uronic acids. The column is then regenerated was 0.5 molar sodium hydroxide to return the column to the hydroxide form prior to the next analytical run. The operating flow rate in this paper was 0.7 ml per minute. Detection was accomplished via Pulsed Amperometric Detection(PAD).