By Chris Nile on Tuesday, May 15, 2001 - 07:33 am:
I am trying to purify small peptides and one of my final steps is size exclusion.The peptides of interest do not absorb at 280nm so i must detect at 215nm.The method i have uses ammonium format however at 215nm this buffer absorbs strongly.I can not use Tris I hope to sequence resulting fractions.Can anyone reccomend a suitable alternative buffer ?
By tom jupille on Tuesday, May 15, 2001 - 03:31 pm:
What pH? phosphate may be an alternative below 3.something or above 7.
If you are purifying, are the peptides present at sufficiently high concentration to use RI detection? That might be the path of least resistance if you already have a working analytical method based on SEC.
Posting is currently disabled in this topic. Contact your discussion moderator for more information.