By Uberto on Sunday, May 20, 2001 - 03:42 am:
Does anyone has the experience working on the analysis of avoparcin, an antibiotic, in animal tissue? I have been trying this using ion-pair chromatography but irreproducible retention time is observed. Any suggestion? Thanks in advance.
By Merlin on Tuesday, May 22, 2001 - 04:55 pm:
What ion-pairing agent are you using? and at what concentration? Most ion pairing agents take an extended period of equilibration to stabilize the retention time. Try using 0.02% nonafluoropentanoic acid (pH ~ 3.0). I have had good sucess with it, and the column is generally equilibrated in 30-60 minutes at 1 mL/min.
By Anonymous on Wednesday, May 23, 2001 - 05:47 am:
Tetra(n)-Butyl Ammonium Hydroxide works.
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