By Anonymous on Tuesday, May 22, 2001 - 12:14 pm:
We currently perform tissue hydrolysis by drying the tissue sample, baking in acid, at low temp, after nitrogen purge.
Does anyone have a different method they use to hydrolyze tissue?
I'm currently analyzing, for lysine, with the Waters ACCTAG AA analysis method and I'm not convinced that my hydrolysis is breaking 100% of the biopolymer chain to release the lysine.
Has anyone hydrolyzed using sodium cyanoborohydride?
By Rachel Smith on Wednesday, May 23, 2001 - 08:36 am:
Anon-
For high recovery, I would suggest a process that was discribed in an article called "Hydrolysis of Proteins and Peptides in a Hermetically Sealed Microcapillary Tube: High Recovery of Labile Amino Acids". Authors are Teh-Yung Liu and Robert A. Boykins, Division of Biochemistry and Biophysics, CBER, FDA. I think it was from Analytical Biochemistry Journal. We use this process quite often. The procedure is very thorough. If you need it I can fax it to you. Email me if you would like it.
What do you think about the Accutag? We are considering the Hitachi L-8800 in 3Q, 2001. We think that the post column ninhydrin method is the best available mode of operation. From the ABRF show, there was an article that studied the differences in methods. Just curious how your results are? Good luck!
By Anonymous on Tuesday, July 17, 2001 - 06:17 am:
We have good results with the acutag method but we have the Alliance system. I really think it's a great system. Thanks
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