By Anonymous on Saturday, July 3, 1999 - 04:31 am:
We are interested in analysing dyes by HPLC. We would appreciate if anyone can suggest a reference for methods for analysing dyes
With Best regards
By de l'Escaille on Monday, July 5, 1999 - 04:43 am:
We have setup some tests for dyes with CE.
By Friedrich Mandel on Wednesday, July 7, 1999 - 04:49 am:
Which chemical class of dyes??
Azo dyes and anthraquinone dyes run nicely on C8 or C18 columns with a water/methanol or water/acetonitrile gradient (10->90% organic). For my LC/MSD experiments I used a 5 mM ammonium formate buffer (pH approx. 3). My preferred column was a Zorbax XDB C8 2.1x50 mm (also 150 mm length possible).
I agree with the previous message. CE is also a good way of separation, in particular with complex matrices. However, LC is more sensitive than CE (Lambert-Beer's law!!).
Best regards
By Anonymous on Friday, July 9, 1999 - 03:57 am:
This refers to the reply of Mr. Friedrich Mandel. We are interested in azo dyes. However if any reference book (like USP for pharmaceuticals) is available it would be very useful. We don't have time and expertise for method development
Best Regards
By Anonymous on Friday, July 9, 1999 - 01:44 pm:
You must consider the kind of sample you want to analyze. For example, if you need to analyze dyes in food, the "bible" for methods is the AOAC manual, or maybe the EPA methods.
Best regards
By Chris Pohl on Monday, July 12, 1999 - 01:44 pm:
Depending upon which azo dyes you're interested in analyzing, methodology has been developed for separating many of these compounds on a multimode (reversed phase/cation exchange) column. Information on the separation is available as part of the OmniPac guidebook available from Dionex. If you are interested in the specifics of the separation or in how to get a copy of the guidebook, please let me know.