By Anonymous on Friday, June 8, 2001 - 12:00 am:
Hello,
can anybody help me with this problem?
At time I use an RP-18 (Nucleosil)and both acids
came at the same time RT:1,0 min.
Thank you
By Anonymous on Saturday, June 9, 2001 - 01:39 am:
Gradient? Isocratic? Mobil Phase? Flow Rate? pH? If you provide some information, you might have a higher probability of getting some help.
By bill tindall on Sunday, June 10, 2001 - 07:18 pm:
Aquasep column from ES Industries. start at 100% water and a bit of phosphoric acid. You are not retaining either under your current conditions, so od course they coelute. Also a piece of cake by gc
By Ivan Vins on Thursday, June 14, 2001 - 04:54 am:
Those acids can be separated by ion exclusion on sulfonated polystyrene-divinylbenzene column in H form, with 1 mM sulphuric acid as an eluent. Contact me for a chromatogram and conditions
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