We have a packed column method which continually gives us trouble with tailing peaks, even on a new column. As several runs are put on the column, the tailing gets much worse and reconditioning of the column may bring it back for a few more runs, but thats about it.

The method uses a glass column (2m x 2mm) with HayesSepQ packing. The analytes are solvents (IPA, Ethanol, ACN, MeOH) and particularly the alcohols tail. The method follows the USP (Section 611) which specifically says "glass column".

I suspect that some of my problem is due to activity issues (especially since ACN does not tail so much compared to the alcohols). I wanted to look into a packed metal column with special deactiviation (i.e. Restek SilicoSmooth).

My question is whether this specially treated metal column can pass for a glass column in the eyes of the USP/FDA?

Also, any suggestions on how to improve the tailing problems of these peaks (also considering that we only have so much wiggle room with the FDA).

Thanks in Advance