By Maja Dimitrovska on Thursday, June 14, 2001 - 03:25 am:
Could someone suggest any simple HPLC method for separation of Potasium Sorbate and Sodium Benzoate in a mixture in food samples, where they are used as preservatives. I need a simple and fast method because I ussualy analyse approx. 10-15 samples a day.
Thanks.
By Anonymous on Thursday, June 14, 2001 - 10:04 am:
We've done Na benzoate by HPLC using 50% ACN and 50% (0.5% H3PO4 in water) on RP-18 column at 1.0 ml/min flow rate and wavelength 273 nm. K sorbate will have less UV absorbance, so less sensitivity. We have assayed both by GC, may want to consider that.
By Anonymous on Friday, June 15, 2001 - 06:43 pm:
Sure. Mobile Phase 20 % ACN and 0.004 m NH4 Acetate Buffer pH 4.4. Column 15 x 4.6 ODS2
such as Spherisorb,Sphericlone. Uv detector:Flow 0.6mL/min
Na Benzoate at 210nm; RT ~ 5 min change wavelength at7 min and Pot Sorbate at < 10 min. At 262nm .If you cannot change wavelength automatically then run twice. it's that quick.
always use guard column Phenomenex the best $ value out there.
Adjust flow for the RT of choice Total analysis time < 12 minutes usually . Store column with 15 % ACN/H2O. do not leave buffer in column without flow
Posting is currently disabled in this topic. Contact your discussion moderator for more information.