By Anonymous on Tuesday, July 6, 1999 - 02:20 am:
I'm using LC (Shimadzu 6-A) to analyze the microbial degradation of 2-chloroaniline, 3-chloroaniline, and 3,4-dichloroaniline.
The LC condition I currently use is :
eluent : methanol-water 50:50 (V/V)
Column : ODS (15 cm)
flowrate : 1 ml/min
Methanol pressure : 110 kgf/cm2
Water Pressure : 60 kgf/cm2
Oven temperature : 30 C
Wave : 254 nm
The result I get :
2-CA, retention time : 4 min
3-CA, retention time : 4 min
3,4-DCA, retention time : 6 min
Is the above LC condition good enough ?
Or anybody have any experience with similar case?
To quantify the chloroaniline degradation I intend to use Aniline as the internal standar. My problem is the retention time of aniline is 3 min,
too close with the retention time of 2-CA and 3-CA. Is there any compound
that can be used as an internal standar for the analysis of CA ?
Thank you
Diatherman Anggen
therman@mail.com
By Justin ( - 199.72.121.89) on Monday, July 19, 1999 - 12:56 pm:
I have worked with a method for para-chloroaniline that utilized GC (ECD) with a derrivatizing procedure. This may be more change than you wanted, but it is an option.
By Anonymous (sfr-qbu-pqm-vty30.as.wcom.net - 216.192.55.30) on Monday, July 19, 1999 - 01:31 pm:
You might consider dropping the organic solvent concentration by about 5% (to 45/55). That should increase your retention times by 40% or so and improve the resolution for the early peaks.
If you need an alternative internal standard, you might look at any of the alkyl derivatives of aniline. You'd have to try them to find one that elutes in a "hole" on you chromatogram
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