By Anonymous on Monday, June 25, 2001 - 11:32 am:
Bases, or more often acids, are added to huamn plasma samples to get ride of protein binding and facilitate extraction. I am curious what causes protein binding. Any types of receptor-drug interaction or antigen-antibody? What about chemical forces between proteins and drugs, e.g. hydrogen bonding, hydrophobic interaction, interaction of opposite charged protein and drug molecules?
Thank you very much for your kind help!
By H W Mueller on Tuesday, June 26, 2001 - 12:16 am:
It looks like all binding to proteins can be explained by known physico-chemical interactions.
For separating (extracting) analytes from proteins one needs to provide a medium that can SOLVATE the analyte, not only a denaturant like an acid. See J. Chrom. B, 678, 137 (1996).
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