By Michael J Oshinski on Friday, April 30, 1999 - 05:53 am:
I am looking for information regarding chiral chromatography method development and theory. I have been involved in LC development for 7 years but have little experience developing chiral procedures. Could you please forward me any literature references and/or training course information? Thank you
Michael J Oshinski
Analytical Chemist
Wyckoff Chemical Company
By Brian Paasch on Monday, May 3, 1999 - 03:14 pm:
While the principles of chiral separation are fairly straightforward, the applications sometimes border on magic. I sat through a seminar last week given by an experienced chiral chromatographer who showed an example of a method where he saw no separation versus great separation of enantiomers on a column that had been simply pre-washed with two different grades of ethanol! (And at the time of his discovery, there was no ethanol in the mobile phase, though he added it after making this observation.) At the end, he sorta shrugged his shoulders and said "don't ask me why, but that is what finally worked."
Most general chromatography text books will have a chapter or so covering the basics (e.g. Practical HPLC Method Development by Snyder, Glajch and Kirkland, 1988). But because there are so many variations and tricks to the methodology, I believe the best source of practical method development information comes from chiral column vendors. Our lab typically uses columns from Astec (973 428 9080), Chiral Tech Inc (800 624 4725) and Regis Tech Inc (800 323 8144) and all three of these companies have considerable "method development" literature. And in our experience, column companies that specialize in chiral separations, often have a competent tech support staff.
Don't overlook affinity separations! If you have access to a protein that you know your molecule binds to, affinity separations may be sensitive to the different enantiomers. A classic example is the separation of enantiomers of ibuprofen on human albumin. Mobile phase is a no-brainer PBS and provides baseline resolution of the two enantiomers, though the run time may be ridiculously long depending on column dimensions and flow rate.
By b.buglio on Sunday, May 16, 1999 - 06:05 pm:
Also see new book "Chiral Chromatography", RPW Scott and T.Beesley, J.Wiley for theory and method development.
By Janusz Debowski on Friday, May 21, 1999 - 01:17 am:
Anybody has done chiral separation of tartaric acid ? Do not tell me to contact Louis Pasteur's ghost, please.
Janusz Debowski
CSIR , Bio/Chemtek
South Africa