By Anonymous on Monday, July 2, 2001 - 01:58 am:
In a preliminary phase of an LC separation of natural components, I have used a single standard in 100% methanol and a 100% methanol mobile phase.
The column used is aC18 – 250*4.6 mm and flow was 1.0 mL/min.
Surprisingly, when the flow is setted to 0.5 mL/min, the same amount injected give a peak of component doubled in area response.
Someone have an explanation for this behavior? Thanks
By K. Kolodsick on Monday, July 2, 2001 - 05:23 am:
The "peak" is moving more slowly through the detector so the detector "sees" it for a longer time, giving it a bigger area. See the link below for a discussion of flow rate vs. peak areas.
http://www.lcresources.com/discus/messages/129/1328.html?FridayJanuary520010349am
By kostas on Monday, July 2, 2001 - 06:53 am:
You can also see the discussion entitled:
"Should Flow Rate Effect Detector Response Like This?"
which took place the 13 March 2001 (until 4 April)
By Anonymous on Wednesday, July 4, 2001 - 02:37 am:
Thanks for your replies
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