By maggie mcmanus on Tuesday, July 10, 2001 - 10:21 am:
Does anyone test for Neo Heliopan Hydro as an active ingredient using HPLC??? We're having problems with it crystalizing out in organic solvents. Any help would be greatly appreciated!
***Needed yesterday! ;)***
Thanks.
By Molever on Tuesday, July 10, 2001 - 04:03 pm:
OK, Mag, I can tell you what we've used for HPLC assay of phenylbenzimidazole sulfonic acid. 10 cm 5 5 micron RP-18 column coupled with 3 cm 5 micron RP-18 guard column Mobile phase A = 4% acetic acid in HPLC H2O
Mobile phase B = HPLC methanol
% B Mobile phase = 20
Mobile phase flow rate = 1.00 ml/min. for 4.6 mm i.d. columns, 0.25 ml/min. for 2.1 mm i.d. columns
Wavelength = 303 nm
With these conditions, PBSA eluted at about 3 minutes. Samples and standard were made up in methanol-water-ammonium hydroxide solution. E-mail me and I can send a Word document of our 1993 test method, as this would not be considered proprietary.
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