Dear all,
I am considering the purchase of a counter current chromatography system, and seek the
advice of the experienced...
How much (mgs? grs?) can be loaded practically during one run?
What would be the expected purity of a substance, injected (loaded) as a crude
preparation (plant extract for instance)?
How many mgs can be expected of such a compound in one run?
Can a CCC system be integrated into an existing HPLC system, and do I have to use a
preparative cell ((in the detector)? Any other con's and pro's ?
Thanks

hey im doing a lab report which is pretty much impossible without a good website dealing with chromatography or Rf values... do you know of any good sites dealing with either of the two?

There are these things called libraries. They are big buildings with a lot of books. You might be able to find one on campus.

To the first anon--sorry, I don't know anything about countercurrent chromatography.

About CCC: We have a CCC system, and I have not been overly impressed with it. It's okay if you are trying to separate compounds that are easily resolved. The main peak will be very broad and will swallow up any close impurities with similar retention times. I've used ours on a Waters prep HPLC (just hooked it in where the column usually goes) system with no trouble.

One major problem that we've had with CCC is method development. You must have two immiscible phases that your compound is equally soluble in, and the density of these two phases must be very different in order for CCC to work. If you have a method, great; if you don't have a method, you've got alot to do.