By Daren on Thursday, July 19, 2001 - 07:35 am:
I'm interested in extracting liposomes from a biological sample and analyzing them for their phospholipid content. I have an HPLC/ELSD method for the analysis of the phospholipid but all the sample prep is in methanol. I need a way to break down the liposome and keep it soluble, the only idea I have come up with is using a roto vap for the samples then reconstituting in methanol and spinning or filtering out the proteins that precipitate out. I don't have a roto vap yet and would like to know if anyone has any ideas or experience with this?
Thanks
Daren
By H W Mueller on Thursday, July 19, 2001 - 11:26 pm:
Most likely, the best method for lipid extraction is the method by Bligh and Dyer. See the GC Message Board, "Fatty Acid Analysis + SPE, ~Feb. 20, 2001.
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