By Anonymous on Tuesday, August 7, 2001 - 06:47 am:
Dear boardreaders,
I am searching for a method for quantification of isoflavones in soy products with an oily matrix by HPLC.
Thanks a lot in advance.
By Anonymous on Tuesday, August 7, 2001 - 07:36 am:
The literature abounds with references and methods for these compounds. I have included but 1.
Improved methods for the extraction and analysis of isoflavones from soy-containing foods and nutritional supplements by reversed-phase high-performance liquid chromatography and liquid chromatography-mass spectrometry
Author: Griffith, Aaron P.; Collison, Mark W.
Source: J. Chromatogr. A / Year: 2001 / Volume: 913 /Page: 397-413
By Anonymous on Tuesday, August 7, 2001 - 11:55 pm:
Thanks alot Anon.
By Anonymous on Wednesday, October 31, 2001 - 07:01 am:
you can use gradioent system
column symmetry c18
flow rate 1 ml/min
mobile phase A 999WATER : 1 HAC
B METHANOL : 899 :10 WATER: 1 HAC
GRADIENT B CONC 0.01 20%
2.01 30.0%
20.0 70%
30 30%
40.0 20%
HOPE YOU WILL END UP WITH GOOD SEPERATION
WAVELENGTH 260 NM
SAMPLE PREPARATION IN 80% METHANOL AND 20% WATER
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