By Vojtech Zabka on Monday, August 13, 2001 - 10:56 pm:
Hello, could anyone help to select a handbook for normal-phase HPLC? Everething seems to be RP-oriented. I need to qualify mixtures of nonionic surfactants and normal-phase might be one of ways, but I would need to learn more than just basics. Thanks.
By Gerhard Kratz on Friday, September 7, 2001 - 09:14 am:
Hello,
a good overview you will get in Uwe Neue's book HPLC Columns Theory, Technology, and Practice. The ISBN is 0-471-19037-3 Wiley-VCH, Inc.!
You are right that normal-phase stepped into the background, what's not fair to this "old" technique. Some problems in RP could be easily solved by using a NP column. Examples are compounts who are not retarded on RP phases. Carbon load was increased to solve this problem and other efforts in "new" surface chemistry. Just using a TSK Amid-80 column with acetonitrile and water as mobile phase will solve such a problem.
For your specific application you can go to our home page tosohbiosep.com (and soon also tosohbiosep.de) with a link to our HPLC database in Japan. There you can find some normal phase applications for surfactants (in English).
You also can get additional applications at Shodex.com! But please click for the English version.
Good luck and success
Regards
Gerhard Kratz
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