By Anonymous on Tuesday, August 28, 2001 - 09:35 am:
Dear all,
I have a question regarding the purication of crude synthetic DNA. The quantities are about 3 gram per year and the batch quantities is 0.6g. What is the best way to purify the DNA via RP-HPLC or other analytical method? Could someone give me some advice or recommendation?
Regards,
David 8/29
By Uwe Neue on Tuesday, August 28, 2001 - 03:36 pm:
We have been doing this type of work. If you contact me at the e-mail address below, I'll get you in touch with the right people for a complete discussion of the subject.
Uwe_Neue@Waters.com
By Tom M. on Tuesday, August 28, 2001 - 09:05 pm:
Are these oligos or long chains of DNA. I have separated oligos using a TosoHaas DEAE column with a NaCl gradient to ~1M. I could definately see failure sequences but I don't know if the main peek was a pure peak or an envelope that also contained unresolved n-1 failure sequences. I was performing displacement chromatography experiments at the time, so I wasn't concerned with peak purity. Is the backbone of your DNA native or modified? Phosphorothioates are much more strongly retained on anion exchange, methylphosphonates can be seperated on normal phase. Good Luck.
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