By Anonymous on Friday, August 31, 2001 - 12:58 am:
Hi there!
I want to get some input regarding the choice of wavelenght selection for purity analyses of libraries with LC. The best compromise is probably using a DAD and sum the range of ex. 210-450 nm. If a DAD is not present in the system I suggest to use 220 nm. Some chemists claim that 254 nm is better due to a "cleaner trace" (salt ion etc does not show up).
Any comments? Please!
By Anonymous on Friday, August 31, 2001 - 10:18 am:
A single wavelength can not give you an estimate of purity of a peak. As well, a DAD can only really give the purity estimate of an individual peak (ie, are there co-eluting components within that peak).
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