By ippich on Tuesday, September 4, 2001 - 12:32 am:
I try to determinate different non-polar lipids with HPLC-ELSD using a polar column (diol or cyan) and heptane as mobile phase. How can i get a good separation of paraffin and different wax esters?
By Merlin on Wednesday, September 5, 2001 - 02:00 pm:
Wax esters will elute as a class by normal phase (no separation). You must use reversed phase to separate different wax esters. Try A = MeCN, B = Acetone (or methylene chloride) with a gradient from 20%B to 80%B.
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