IŽd like to analyse the carotenes in plants, especially the three xanthophylls antheraxanthin, lutein and zeaxanthin with RP-HPLC.
Have anyone datas about suited HPLC-columns, instrument parameters and mobile phase?
At the moment I use ACN/H20 and Ethyl acetate. With this methode I cannot differentiate three peaks.
I hope anyone can help.
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By Anonymous on Sunday, November 11, 2001 - 08:23 am:
Chromatographic determination of carotenoids in foods. Oliver, Jordi; Palou, Andreu.
J. Chromatogr. A, 2000, 881, 543-555
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By mc on Friday, November 16, 2001 - 06:01 am:
Maybe the following application note is of help:
Simultaneous Analysis of Carotenoids, Retinoids, Tocopherols, Vitamin K1 and Coenzyme Q10 In Plasma
You can find it under: www.msscientific.de/101176p.htm
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