By Anonymous on Friday, September 7, 2001 - 02:47 am:
I would greatly appreciate the definition of the following terms in LC:(i) Tailing Factor and (ii) Linearity. Finally how are these parmeters measured and interpreted?
By Gerhard Kratz on Friday, September 7, 2001 - 09:56 am:
Hello,
Tailing factor by U.S. Pharmacopeia is the ratio of the width of the peak to 2 times the front of the peak, measured at 5% of the height of the peak: T = f5% + t5% / 2f5%
Linearity - do you mean linear velocity?
This is named u and based on the breakthrough time of an "unretained" peak, t0:
u0 = L / t0
Hope this helps.
Regards
Gerhard Kratz
By Anonymous on Friday, September 7, 2001 - 03:34 pm:
Can anyone explain how Linearity is measured when a sample is run with standards?
Many thanks.
By Anonymous on Friday, September 7, 2001 - 08:23 pm:
Many people use regression analysis to measure the linearity of a standard curve. Go to any search engine and search for "Regression Analysis".
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