By shirish on Thursday, October 25, 2001 - 12:03 am:
I AM TRYING TO DEVELOP A HPLC METHOD FOR THE ACARBOSE BY ECD DETECTION AS I FOUND THAT THIS IS ELECTROACTIVE COMPOUND I TIRED WITH L29 COLUMN AND M.P IS 70 BUFFER KH2PO4 AND 30 ACN PH OF M.P IS 11.0 I GOT THE RESPONSE AND PEAK AT 6.5 MINUTES THIS IS ALUMINA COLUMN NOW THIS WAS THE M.P OF AZITHROMYCINE METHOD . WORKING WITH WATERS ECD AND E1 IS 700 AND E2 IS 820 AND WORKING IN A 2P STAT MODE .
I FOUND THAT THIS COMPUND IS STABLE AT 6.7 PH . AND THIS TYPE OF COMPOUND WIIL ELUATE BETTER IN A
AMINO COLUMN SO HOW ATO OPTIMIZE THE PEAK RESPONSE IN A ECD THAT I WANT TO KNOW
IF ANYONE HAS ANSWER CAN DIRECTLY CONTACT ME AT
shi_ri@yahoo.com
By Ed Farmer on Thursday, October 25, 2001 - 08:36 am:
I assume that ACARBOSE is a type of sugar. You will need to use PULSE amperiometric detector (PAD), as the electro chemical detector at fixed voltage gold electrode will become coated with sugar. If you use a ECD, the second and subsequent injections will give smaller peakes for the same concentration injected. You must use a PAD to regenerate and clean the gold electrode surface. You can refer to the Metrohm Bioscan see Metrohm.com web site for application of sugars. Eluents tend to be 50mM NaOH and with NaAcetate xxmM concentrations.
good luck Ed
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