By anna on Thursday, October 25, 2001 - 12:31 pm:
I`m troubling with strange baseline and chromatogram even for testmix. I``ve just changed to a new LC-318 column, before I used a LC-18. Does that make the big different`.Or could it be contamination. I use the old guide-column. I run with recommened conditions. The peaks are small and broad.I`d looked for leakages. Everything seems to go wrong.
I`m frustareted
By Anonymous on Friday, October 26, 2001 - 01:18 am:
more info please.
"strange" baseline?
"recommened" conditionns?
"everything" seems to go wrong?
what are you analyzing? equipment (detector, mobile phase etc)?
lc-318 column?
this question can never be answered!
By Dave on Wednesday, March 20, 2002 - 06:30 am:
Anna... Never touch an LC again.
By Jim Gorum on Wednesday, March 20, 2002 - 07:48 pm:
Anna,
There's another discussion about converting from 100A pore to 300A pore columns is that what you have done?
By Will on Thursday, March 21, 2002 - 01:01 am:
Bit harsh there Dave old chap!
Anna, as anonymous said, give us as much
information as you can about your system,
and we'll see what we can do.
How old is the kit you are using?
Plumbing the bits together is not as simple as
it looks. Bad connections lead to not just
leaks, but dead volumes can mess up the
separation.
Good luck!
Posting is currently disabled in this topic. Contact your discussion moderator for more information.