My project involves running 2 chromatography steps to separate digested peptides. The latter has to be Reverse phase chromatography, could anyone suggest any relavant material or sources of references that i could look at to help me, please..

Look on the: http://www.biaseparations.com/app/App_004.htm. They have been using two different modes in one column. Perhaps you can build up a similar column using RP instead the Protein A disk.