Retention Factor

Chromatography Forum: LC Archives: Retention Factor
Top of pagePrevious messageNext messageBottom of pageLink to this message  By Austin Li on Thursday, November 8, 2001 - 05:32 am:

I read of a paper mentioned about a term Retention Factor k. I am not sure what this term means. Does it mean capacity factor? Any suggestion is highly appreciated.

Austin


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Thursday, November 8, 2001 - 07:39 am:

Retention Factor k = (tR-t0)/t0

tR= Band Retention Time
t0= Column Dead Volume Time

If I am not mistaken, k'(capacity factor) is the same thing.


Top of pagePrevious messageNext messageBottom of pageLink to this message  By jclark on Thursday, November 8, 2001 - 08:22 am:

k' and k are the same thing. IUPAC codified the terminology several yrs ago.


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Austin Li on Thursday, November 8, 2001 - 12:13 pm:

Thanks a lot for the contribution from Anonymous collegue and jclark.


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Monday, November 12, 2001 - 05:59 pm:

OK. Now what about relative retention time? How is this calculated?


Top of pagePrevious messageNext messageBottom of pageLink to this message  By David Blais on Tuesday, November 13, 2001 - 08:21 am:

Relative retention time is simply the ratio of the retention time of a "secondary peak" to the retention time of a "main peak." For example, you wish to calculate the RRT of formic acid ("secondary peak", retention time of 7.0 minutes) relative to malic acid ("main peak", retention time of 2.0 minutes). The RRT of formic acid would be 7.0/2.0 = 3.5. Now, if malic acid were the "secondary peak" and formic acid were the "main peak," using the same retention times, the RRT of malic acid would be 2.0/7.0 ~ 0.29. I hope this helps.


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