By kati on Monday, November 12, 2001 - 01:58 pm:
We currently use a RP HPLC method that has DMF and EDTA in its mobile phase (oxalate buffer, THF, DMF and EDTA). Reading about method development I have never found mentioning DMF or EDTA as additivs to the mobile phases. What colud be the reason of using them?
By bill tindall on Monday, November 12, 2001 - 03:31 pm:
Without any information on what you are separating,pH, or the concentration of these components, it is not obvious what these components might be contributing to the separation. You might try leaving them out, one at a time, and see what happens. If something happens, it will become clear why they are there.
By jclark on Tuesday, November 13, 2001 - 05:12 am:
EDTA is used to chelate metals in silica to eliminate their sometimes severe effects on sensitive analytes.
By Anonymous on Sunday, November 18, 2001 - 01:12 pm:
EDTA can be used to save analytes that complex with the metal in your HPLC system. DMF is not used very much because of the high viscosity.
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