By Kevin Gordon on Monday, November 26, 2001 - 05:28 am:
I am having difficulty developing a method for the analysis of DMSO (circa 3%) in water. I wonder if anyone may have some advice on how to proceed. Evidence gained so far, has established that DMSO is the only contaminant.
By bill tindall on Monday, November 26, 2001 - 03:07 pm:
I have never had need to determine DMSO, but DMSO/water has been a solvent in things I have analyzed. From this experience I know that it can be retained on a Aquasep column (ES Industries) and probably every other "aqueous" column and it gives a good UV absorbance signal. I don't know what the peak shape will be because in my cases it was always overloaded, but I don't see any reason that there would be a problem.
By Ken Norris on Wednesday, November 28, 2001 - 10:52 am:
I have been able to assay DMSO using a Zorbax column RX-C8 5 micron. With a mobile phase of 0.01% NaCl, 0.1% H3PO4. It is only retained for 3.5 minutes at a flow rate of 1 mL/min. LOQ is about 0.05%
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