Increased Capacity with methanol

Chromatography Forum: LC Archives: Increased Capacity with methanol
Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Saturday, December 8, 2001 - 05:11 am:

Hi,
I am using Amberchrom CG 161 resin for capture of a product at a very high linear velocity. When I added 10% methanol to the aqeous stream, there appear to be over three fold invrease in the columns capacity. I'd like to point out that there is about three fold increase of the product's concentration in the feed when the 10% methanol was added (product has low solubility in water). Am I correct in assuming that the increased concentration, plus improved mass transfer (viscosity contribution), especailly when we are flowing at a very high linear velocity, may have much to do with the increased capacity. My collugues think that I completely wrong, and that the increased ellutropic solvent strength by the addition of the 10% methanol should be enough to offset any gains from increased concentration and improved mass transfer. Can you anyone help me understand the rational behind the three fold capacity increase.


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