By Tina on Wednesday, December 19, 2001 - 11:27 am:
For some reason two compounds that are very similar (the only difference is a single methyl group) have very different resolutions. I am doing 40/40/20 gradient using MeOH, H2O, and ACN. The two compunds that are giving such a problem are glyoxal and methylglyoxal. No on in my task group can figure out the drastic difference in retention as the concentrations increase in the calibration standards. Any advice or help would be greatly appreciated!
By gen on Wednesday, December 19, 2001 - 11:53 am:
Tina, you mentioned gradient...... from 40/40/20 to ??? What kind of column? Sounds like the componds of interest are well seperated and you should be very happy. I am not sure what your problem is. Please, we need more info.
By Anonymous on Saturday, December 22, 2001 - 06:55 am:
you should increase tea in your mobile phase or add tetra butyl ammmonitu hydroxide in your mobile phae
By Anonymous on Monday, December 31, 2001 - 07:27 am:
The unusual properties of methylglyoxal are
discussed at length in Chapter 3 of THE LIVING
STATE AND CANCER by Albert Szent-Gyorgyi,
second edition (Marcel Dekker, 1978).
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