By leea ( - 194.102.146.67) on Wednesday, July 21, 1999 - 07:17 am:
It happen few times that the tail of a peak is not coming back at the baseline, remains at about 10% of the peak height. On the next injection was the same. It was not so bothering, but I'm keep asking why? Does anyone face with this kind of problems?
By hinsbarlab (dyn1-tnt13-142.detroit.mi.ameritech.net - 199.179.188.142) on Wednesday, July 21, 1999 - 08:44 am:
Without more infomation I would guess that either:
1. You have a compound that is strongly retained on the column and and is being slowly washed off after the injection and/or
2. You are overloading the column.
If you let the run continue for a long time, does the baseline ever return to its original height? Does this happen with every injection or only occasionally? Does it happen with new or old columns or both? Does it happen with standards or only with samples.
Please respond with more specifics about the analysis (mobile phase, sample concentration, absorbance levels, etc.).
Regards,
Michael Hinsberg
Hinsbar Laboratories, Inc.
www.hinsbarlabs.com
By lisa (spider-tl083.proxy.aol.com - 152.163.207.213) on Wednesday, July 21, 1999 - 01:15 pm:
I had this problem once also, turned out that the pH of my mobile phase was right on the pK of the drug I was looking at.
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