By awolff on Thursday, December 20, 2001 - 06:48 am:
I am having problems with carryover. It's specific to a certain method we are using. We are having constant carryover (that is very minimal) but if the HPLC sits idle (with mobile phase running) for a long period of time, the first injection after the idle has a very large amount of carryover. Does anyone have any suggestions? I've tried checking fittings and increasing the wash solvent strength.
By dr on Thursday, December 20, 2001 - 01:24 pm:
Isocratic, or gradient?
By Anonymous on Sunday, March 17, 2002 - 06:33 am:
This quite obviously suggests you have contamination in the mobile phase.
The reason why you are getting a larger amount of "carryover" after the system has been sat idle for a while suggests that you are getting a build up of contamination as it sticks to the column so when you run the gradient through the contaminant goes through.
I suggest you make a new batch of mobile phase and this should improve things.
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