By Anonymous on Thursday, January 3, 2002 - 06:01 am:
I AM LOOKING FOR HPLC METHOD OF ANALYSIS FOR
ERYTHROMYCINE CYCLO-11, 12- CARBONIUM .
By Anonymous on Friday, January 18, 2002 - 04:32 am:
okey i am telling you what work i have done
]
i have strated work with the ECD DETECTION AS THIS COMPOUND IS HAVING VERY POOR UV ABSORBANCE
MOBILE PHASE IS ( 70 BUFFER KH2PO4(0.05M) PH 11 WITH 10% NAOH AND ACN 30 )
COLUMN IS L 29 COLUMN
FLOW RATE IS 1.0 ML/ MIN
E1 IS 700
E2 IS 820
i am getting peak at 12 minutes that is very broad
plates is 300 only but tailing is 1.05 so peak is gaussian
so how to improve on this performance
By Anonymous on Friday, January 18, 2002 - 02:58 pm:
Whats an L29?
By Anonymous on Sunday, January 20, 2002 - 01:33 am:
L29 USP COLUMN
GAMMA ALUMINA RP18
Posting is currently disabled in this topic. Contact your discussion moderator for more information.