By Anonymous on Friday, January 11, 2002 - 12:16 am:
i´d like to know what kind of material is better to the analysis of betalactamic antibecterials with basic properties to get a good peak shape.Thanks.
By KAF on Friday, January 11, 2002 - 12:46 am:
I would begin with a BDS-C18.
Conditions will depend on solubility, pK of the sample, stability at different pH ,.....
You can try to find a similar structure in the USP or EP to have the first aproach.
KAF
By Michele on Friday, January 11, 2002 - 05:46 am:
I have used both C8 and C18 with 50/50 ACN/MeOH organic phase and formic acid/ammonium formate buffer. I don't remember the gradients exactly.
By Anonymous on Friday, January 11, 2002 - 07:53 am:
To Anon,
The newer Waters Xterra columns show very good properties (i.e. peak shape) when analyzing basic compounds and have the added advantage that they are fairly stable and have good lifetimes at high pH values of mobile phases which are sometimes used to help with peak shapes of basic compounds.
Regards,
Mark
P.S. - No I don't work for Waters.
By MFB on Friday, January 11, 2002 - 08:46 am:
I've used Luna C18(2)(Phenomenex) and Merck Purospher for basic drugs (pseudoephedrine, clorpheniramine, oxybutynin, etc.) without need of TEA or other additives, just KH2PO4 15-30 mM ph=3-7 + organic solvent (MeOH - MeCN) depending on the specific application.
By Anonymous on Friday, January 11, 2002 - 10:46 am:
I've had lots of luck analyzing basic compounds using a ZirChrom-PBD column with acetonitrile : ammonium phosphate buffer mobile phase.
By Anonymous on Thursday, February 28, 2002 - 04:16 am:
thank you all .
thankyou very much...I´ll try some of them
By Anonymous on Monday, March 4, 2002 - 01:41 pm:
There are many Zorbax columns that can separate basic columns. zorbax is an Agilent technology product
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