By Jason Cameron on Friday, January 11, 2002 - 08:49 am:
I am looking to analyse samples of sulphonated dyes (similar to Cibracron blue F3G-A) by vis-HPLC. I was wandering if they can be analysed without using an ion pair reagent. If so do I need to keep the pH low enough to protonate the sulphate groups or can I get away with running them ionised?
By Molever on Friday, January 11, 2002 - 10:08 am:
We've done FDC Blue #1 by HPLC using 0.01 M perchlorate at pH 3 on RP-8 column. See L. Gagliardi, G. Cavazzutti, A. Amato, A. Basili, and D. Tonelli, “Identification of Cosmetic Dyes by Ion-Pair Reversed-Phase High-Performance Liquid Chromatography,” Journal of Chromatography, 394 (1987), pp. 345-352. If this doesn't help, E-mail me.
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