Hello
In my current project I am developing a method to separate 8 impurities and an active ingredient for a pharmaceutical product. There are two BP methods to separate all 8 impurities for this particular product and both uses a amide column with the following mobile phase.
Method 1: 65% H2O:1% (4% diehylamine):34% CH3CN
Method 2: 65% CH3CN:1% (4% diethylamine):34% H2O
If I prepare this mobile phase with diethylamine, the pH is around 12 or so. Can amide columns survive such a high pH? I have been using it for a while and I don't seem to have any problem.

Hello,

I wouldnt know the exact answer to your question, but you can check for the pH range in which you cna use the column. If the data sheet tha came with the column says the column can survive at that pH, then it, if not, the your column will have a short life time. Agilent Technologies has a column that can survive that pH region, but I dont know if it will be suitable for you. The Column is called "Zorbax Extend"