By Anonymous on Wednesday, February 6, 2002 - 04:54 am:
I AM GO NA TO DO A METHOD VALIDATION STUDY FOR MY BULK .
BUT AFTER DOING INJECTION OF STD . IAM GETTING PEAK PURITY ANGLE IS LARGER THEN THRESOLD VALUES. BUT WHEN I HVE TRIED TO SEE THE SPECTARA AT EACH POINT TO THE PEAK ITS SHOW THE SAME SPECTERA AS MY APEX SO WHAT IS THE PROBLEAM IS REALLY THE MY PEAK IS IMPURE EVEN THOUGH IT SHOWS THE PEAK HOMOGINITY IN TERMS OF SPECTRA .
By Anonymous on Wednesday, February 6, 2002 - 05:13 am:
Spectra that my seem very similar to you manual examination can be (and often are) discriminated by peak purity analysis.
By Anonymous on Saturday, March 30, 2002 - 10:18 am:
Have you set your measurement of the noise at a good place, if this isn't it is posible you can find not pure stnd peaks.
By Anonymous on Sunday, July 28, 2002 - 07:33 am:
hi
About your peak purity i can say that waters 996 detector is linear up to 0.8 aU so if your peak response is over 0.8 then reduce your concentration. Make it half and check the purity i hope it will pass your peak purity. The other way to check that there is no interference from any other degradent/impurities check the spectra. You can also do one thing instead of full range u can check using +- 25 nm from your suggested wavelength and u will find the peak purity.
By haveanopion on Sunday, July 28, 2002 - 12:40 pm:
Peak Purity is horse hockey. The software is arbitrary and capricious as to the threshold angle. If the spectra look the same they probably are. This is a bunch of pseudoscience that has been thrust on us by instrument manufacturers that have lobbied inexperienced reviewers at the FDA. If you really want to know about your separation you will need to run an orthogonal method or capture the elution volume about the main peak and separate it on an orthogonal method.
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