By Anonymous on Wednesday, April 3, 2002 - 05:48 am:
We work on drugs and metabolites in human plasma by LC-MS/MS. It happens sometimes that we have sample contamination during extraction even after throughly cleaing all pipettes and benches with IPA. The contamination is usually severe for LC-MS/MS methods which usually have very low LOQ and we have never had problems with HPLC methods. I am curious what causes such contamination? How should we deal with those "sticky" molecules?
Thank you very much for sharing your thought and experience.
By Anonymous on Friday, April 5, 2002 - 09:20 pm:
What kind of drugs are you analyzing? It is because we seem to have such a problem too.
By H W Mueller on Monday, April 8, 2002 - 11:55 pm:
You seem to be describing carry-over which has been discussed before. Maybe you did not correctly clean the syringe or injection device??
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