By Anonymous on Friday, April 12, 2002 - 02:54 am:
I want to seperate all amino acids using reverse phase hplc and using uv vis detector can any one help.
By Anonymous on Friday, April 12, 2002 - 07:54 am:
Have you tried anything, because it is realy easy to put a question onto Chromforum and let the otherones do the job !!!!!!!!
By bill tindall on Friday, April 12, 2002 - 08:37 pm:
I've done hundreds of bovine serum amino acid analyses and could help. Want my consulting address(just kidding, this forum isn't for advertising)? You are asking for a very large amount of information to do this analysis well, especially for all amino acids. Better start with the literature and allow a few months to learn the field. This was the most difficult analysis I ever did by lc.
By Anonymous on Sunday, April 14, 2002 - 10:53 pm:
Mr. Bill Tindall,
If u have done this work then u can pass some important tips to me so it will save my time as i will not repeat all the experiments and i will be really thankful to u.
thanks
By David Blais on Monday, April 15, 2002 - 11:55 am:
Anon #1,
When I used to perform AAA by HPLC I would use Waters AccQ-Tag system. Visit their website at
http://www.waters.com/watersdivision/waters_website/Applications/accqtag/acqtop.asp
to get more information.
While this method is designed for fluorescence detection, I would also collect on a UV channel without any difficulty. In fact, UV detection is necessary for visualization of tryptophan.
Good luck, I hope this helps.
By Vivek on Monday, April 15, 2002 - 10:19 pm:
Thanks Anon,
I have already visited there site. I Was planning for a seperate method. Again thanks for the suggestion.
By Kostas Petritis on Tuesday, April 16, 2002 - 05:43 pm:
Vivek,
When you say all the amino acids I assume you speak about all the proteogenic ones (or physiological as well)? And when you say "I was planning for a seperate method" do you mean that you are trying to develop a new LC method for amino acid analysis or just you make reference to another derivatization chemistry and if yes which one?
By Vivek on Tuesday, April 16, 2002 - 10:19 pm:
Kostas,
I mean all proteogenic ones. I want a easy derivitization step (pre column ) and stable one (as i am not having precolumn derivitization accessories which r with most HPLC system)to be analysed using UV-VIS or PDA detector.
I want the procedure for PTH derivitization and also want information on DABS column from supelco. In their website they say nothing about this column properties.
By Silvio10 on Friday, September 27, 2002 - 02:59 pm:
Recently I standarized this one. The method employ two derivatization reagents those are Ortho phtaldehyde and also fluorenyl-methyl-chloroformate (FMOC AND OPA) and you need two detectors too.There are some information in Food analysis by HPLC (Nollet et-al).
I hope this help you.
Until then.
Silvio Antonio Ayala Lopera.
Chemist.