By valldev on Thursday, May 2, 2002 - 07:57 am:
While dansyl chloride derivatization works well for HPLC determination of primary amines, can anyone tell me for sure if it will work for a secondary amine? Or for that matter a tertiary amine as well? Thanks so much.
By tom jupille on Thursday, May 2, 2002 - 12:39 pm:
It's been many years since I played with this, but:
"As an aromatic sulfonyl chloride, Dns-Cl reacts with primary and secondary amino groups even at a slightly alkaline pH; at higher pH with phenols, imidazoles, and slowly even with alcohols"
from "Handbook of Derivatives for Chromatography", Blau & King, pg 350; Heyden (1977).
By valldev on Friday, May 3, 2002 - 08:45 am:
Thanks so much Tom
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