By Anonymous on Saturday, May 11, 2002 - 12:53 am:
Hi Friends!
I'm working on "LIRANAFTATE" HPLC method.
I have used a mixture of phosphate buffer (10 M mol) and Methanol (pH 5)on a C18 column. Very bad, the peak is very broad with a long tail. I tried to improve it by changing the pH,but no use.
Can anybody suggest furtherimprovement of this method or an alternative method?
By A.Nonymous on Saturday, May 11, 2002 - 11:11 am:
There are special base-deactivated HPLC columns or specially treaded columns on the market, such as X-Terra, Zorbax, ....
Have you tried this already?
An other approach is to use a pH above 7, this isn't going with all the columns (X-Terra allows pH 1 - 12 ).
Question: Do you bring the buffer to pH 5 or the mixture.
If you bring the mixture to pH 5, you shouldn't, you actually don't know the real pH of your aqueous pH, it is really better to bring the buffer to pH 5 and then mix.
Lot of succes !!!!
By Anonymous on Sunday, May 12, 2002 - 07:25 pm:
Also, phosphate is not a very good buffer at pH 5 !
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