Urgent!

Chromatography Forum: LC Archives: Urgent!
Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Wednesday, May 15, 2002 - 03:43 am:

Hello, I´ve made a mistake, and I´d like someone to help me...
I´ve put acetonitrile /water(65:35), over hexane into a detector..
Now I don´t know how to get a baseline ...And how to clean it
Can anyone hel me?
Thanks....


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Wednesday, May 15, 2002 - 04:08 am:

I would flush the system with IPA. ACN, Water and hexane are all soluble in IPA.


Top of pagePrevious messageNext messageBottom of pageLink to this message  By juddc on Wednesday, May 15, 2002 - 07:41 am:

I'll second that...run it through with IPA and re-eqilibrate with your mobile phase and all should be well. Run your pump slowly, though, as IPA is quite viscous and will result in high back pressures, especially when mixed with water at room temperature.


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Wednesday, May 15, 2002 - 09:48 am:

Thank you very much,I´m realy grateful...


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