By Anonymous on Wednesday, May 22, 2002 - 10:36 am:
i have a sample that is short (7-8 C atom) alkyl chain diastereomers, with no aromatic rings.
have tried to separate on both ODS and Hypercarb column with no success. i figured the chain structure make it not rigid at all, so it's hard to fit any mechanism to separation.
any idea?
seems to me most time for such separation is just try and error, any guideline practically useful? most books just talk about general.
By Anonymous on Wednesday, May 22, 2002 - 03:14 pm:
methanol-acetonitrile-THF.. Any difference?
By Anonymous on Thursday, May 23, 2002 - 05:12 am:
tried that, not much difference,THF is the strongest, peak comes out earlier, but no separation.
By Anonymous on Thursday, May 23, 2002 - 07:11 am:
What about C27 or C30?
By Anonymous on Thursday, May 23, 2002 - 08:02 am:
Maybe one of the cyclodextrin bonded phases?
By Anonymous on Thursday, May 23, 2002 - 05:19 pm:
maybe normal phase on silica is better after all
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