By Anonymous on Monday, June 3, 2002 - 07:46 am:
How HPLC detector flowcell's pathlength & flowcell volume affects the response to a particular component?
By Anonymous on Monday, June 3, 2002 - 09:30 am:
the absorbtion for a compound is directly proportional to the pathlength that the light travels throught the compound (within limits) -Beers Law.
Check a general chemistry textbook for a full description.
By Anonymous on Tuesday, June 4, 2002 - 01:27 pm:
And depending on what you mean by "response" the volume of the flow cell will affect the peak width of the elyuted componenet. The greater the flow cell volume the more peak dispersion and the wider (lower height) the peak will be. Ever notice that there is seldom a simple answer to any question? ;-)
Mark
By Anonymous on Wednesday, June 5, 2002 - 06:30 am:
If there were simple answers, there probably wouldn't be the question.
By Anonymous on Wednesday, July 3, 2002 - 01:49 pm:
As the submitters said the above is true for absorbance. In fluorescence, electrochemistry etc. where the response is dependent on equations other than Beer's law things change.
liz Robertson