Hello,

is anybody have an general HPLC "method" to determine the aqueous solubility of organic compound ?
Thank you for your responses
Cordially

While the relative retention of particular compounds may roughly correlate to their solubility in water, I think there are too many variables involved to make such a method as you suggest remotely possible. Consider theobromine - not very soluble in water, yet elutes more quickly under mildly acidic Rp conditions than some components with much greater solubility in water, such as methyl paraben. I think it would be a much simpler matter to simply either look up the solubility info you're interested in of just do an old fashioned solubility experiment at the bench. Why make things more complicated than necessary? HPLC's are great flexible instruments, but they won't help you answer every chemistry problem out there.

Sorry...

Chris

There are many researcher who have looked at the use of HPLC to estimate the relative hydrophobicity of organic compounds. Log P ( partioning of a drug between octanol and water) determination are often done using HPLC. The higher the log P then the lower the solubility in water. This can be useful in drug research and is part of Quantitative Structural Activity Relationships (QSARS). Valco is a name that comes to mind who does a lot of work in this area.