Has someone read any good review articles and/or book chapters on HILIC? If yes, please give reference lists.

I am very interested in such technique for LC-MS and HPLC of biological samples.

Is gradient elution feasible in HILIC? Let's say, from 80% MeCN to 50% MeCN?

Hi Anonymous, a good intro to HILIC you will find in Uwe Neues book HPLC columns, theory, technology and practice, chapter 11! Also you can have a look into the work of Strege, M.A., "Hydrophilic Interaction Chromatography - Electrospray Mass Spectrometry Analysis of Polar Compounds for Natural Product Drug Discovery," Anal. Chem., 70, 2439-2445 (1998). HILIC columns can be used for widely different applications like carbohydrates, oligosaccharides, oligonucleotides, peptides, nucleic acids, peptides and all other polar compounds, as well as polar pharmaceuticals. HILIC columns can be used with gradients. No problem. At HPLC 2002 in Montréal one of our US customers told me that he is using our HILIC TSKgel Amide-80 column for polar compounds and proteins with gradient elution and ESI-MS and LC-MS-MS (Pharmacokinetics) without any problems, like baseline noice or unspecific peaks. The only "problem" he had was, that column lifetime is too long, to be a "good" customer (just kidding!). I will be more than happy to send out an abstract on this HILIC phase, together with a reference list. Just e-mail to g.kratz@tosohbiosep.de! Gerhard

Dear Anonymous,
In addition to the last posting, I just want to add the following hyperlinks. The first link exemplifies a HILIC separation of a tryptic digest of cytochrome C. At the end of the pdf file you can find 21 references to HILIC litterature published between 1990-2001.

The second link exemplifies a HILIC separation of cationic and hydrophilic peptides. Both these applications are examples of gradient elution in HILIC mode

http://www.sequant.com/pdf/2700-01A.pdf
http://www.sequant.com/pdf/2700-02A.pdf